Generator

Part:BBa_J36849:Design

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-29)

Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6 tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 609
    Illegal AgeI site found at 1143
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no spacer nucleotide between the end of the device and the SpeI restriction site.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts.

Source

Assembly of J04500 and J36845

References